Preparation of Slices
نویسنده
چکیده
homogenates. Some of the limitations of these preparations are: 1) lack of certain inputs and outputs normally existing in the intact brain; 2) certain portions of the sliced tissue, especially the top and bottom surfaces of the slice, are damaged by the slicing action itself; 3) the life span of a brain slice is limited and the tissue gets "older" at a much faster rate than the whole animal; 4) the effects of decapitation ischemia on the viability of the slice are not well understood; 5) Since blood-borne factors may be missing from the artificial bathing medium of the brain slice, they cannot benefit the preparation and thus the optimal composition of the bathing solution is not yet established.
منابع مشابه
Cardiac tissue slices with prolonged survival for in vitro drug safety screening.
INTRODUCTION We have recently introduced the use of mammalian cardiac tissue slices for in vitro drug testing purposes. Here we show how this method can be applied for long-term studies in safety pharmacology. METHODS In freshly prepared cardiac slices from guinea-pig or rat ventricle, extracellular field potentials (FP) and intracellular action potentials (AP) were recorded in response to el...
متن کاملOccurence of dentinal defects after root canal preparation with R-phase, M-Wire and Gold Wire instruments: a micro-CT analysis
BACKGROUND This study aims to evaluate the frequency of dentinal defects after root canal preparation with the ProTaper NEXT, K3XF and WaveOne GOLD systems using microcomputed tomography. METHODS Sixty permanent mandibular incisors with a single canal were selected. Inspection of the teeth was performed under a stereomicroscope (15x) to observe the presence of pre-existing cracks and fracture...
متن کاملNew device for preparing thin slices of adipose tissue for metabolic studies in vitro.
A new microtome is described which allows the rapid preparation of equal slices of well-defined thickness of fresh human tissue, especially adipose tissue. Presetting the microtome for a section thickness of 500 micro m, we found a variation of about 5% with human adipose tissue. Slices of human adipose tissue sliced by the microtome showed a higher sensitivity to insulin and a better reproduci...
متن کاملWarmer preparation of hippocampal slices prevents synapse proliferation that might obscure LTP-related structural plasticity.
The hippocampal slice is a popular model system in which to study the cellular properties of long-term potentiation (LTP). Synaptogenesis induced by exposure to ice-cold artificial cerebrospinal fluid (ACSF), however, raises the concern that morphological correlates of LTP might be obscured, especially in mature slices. Here we demonstrate that preparation of mature hippocampal slices at room t...
متن کاملProtective effect of curcumin and Curcuma longa extract on apoptosis of motor neurons in cultured spinal cord slices of adult mouse
Introduction: Since Curcuma longa extract and curcumin have been shown to be potent antioxidant agents, they were used in cultured adult mouse spinal cord slices to investigate whether they can inhibit apoptosis in motor neurons. Methods: Slices from the thoracic region of adult mice spinal cord were divided into four groups: 1. Freshlyprepared slices (time 0) 2. Control 3. Slices treated by cu...
متن کاملDopamine Modulates Spike Timing-Dependent Plasticity and Action Potential Properties in CA1 Pyramidal Neurons of Acute Rat Hippocampal Slices
Spike timing-dependent plasticity (STDP) is a cellular model of Hebbian synaptic plasticity which is believed to underlie memory formation. In an attempt to establish a STDP paradigm in CA1 of acute hippocampal slices from juvenile rats (P15-20), we found that changes in excitability resulting from different slice preparation protocols correlate with the success of STDP induction. Slice prepara...
متن کامل